%0 Journal Article %T A reversed-phase high performance liquid chromatography approach for analysis of 5-Fluorouracil %J Trends in Pharmaceutical Sciences %I Shiraz University of Medical Sciences %Z 2423-3722 %A Azadi, Soha %A Azadi, Amir %A Ashrafi, Hajar %A Mohammadi-Samani, Soliman %D 2017 %\ 03/01/2017 %V 3 %N 1 %P 49-54 %! A reversed-phase high performance liquid chromatography approach for analysis of 5-Fluorouracil %R %X A reproducible, sensitive, accurate and selective high performance liquid chromatographic (HPLC) method with ultraviolet absorbance detection has been developed and validated to quantitate 5-Fluorouracil (5-FU), an antimetabolite chemotherapeutic agent used in treatment of colorectal cancer as a drug of choice. Chromatographic separations were performed on a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) as the reversed stationary phase, eluted with a mobile phase composed of deionized water and methanol (95:5) with the flow rate of 1.0 mL/min. The UV wavelength was set at 261nm. The method produced linear responses throughout 5-fluorouracil range of 25-500 ng/ml with a correlation coefficient of 0.998. A limit of quantitation (LOQ) was established at 25 ng/ml. The within-day and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests indicate a proper separation efficacy as well as accuracy and sensivity of method which is a potential tool in many pharmaceutical studies such as drug delivery of this anti-cancer agent.  %U https://tips.sums.ac.ir/article_42219_19cf95da5bd7abb6b6e8b47e6da97bff.pdf