Document Type: Research(Original) Article
Myrtus communis L. is a plant traditionally used as an antiseptic and disinfectant drug. In this research, the antioxidant activity of Myrtus communis was assayed by evaluating radical scavenging activity, reducing power, FRAP method and determination of phenolic compounds. The methanolic extract of leaves of Myrtus communis was fractionated by using petroleum ether, chloroform, ethyl acetate and buthanol. In reducing power, different concentrations of samples were mixed with phosphate buffer, ferrocyanate, TCA and ferric chloride. Different concentrations of samples were mixed with DPPH and after 30 min the absorbances were measured. For determination of phenolic content, 500 µl of sample was mixed with Folin-Ciocalteu and sodium carbonate. For determination of flavonoids, 500 µl of sample was mixed with 2 ml of distilled water, NaNO2 and NaOH. In reducing power method, chloroform fraction showed the highest reducing capacity. In the DPPH radical scavenging method, the highest antioxidant capacity was found in buthanol fraction (IC50=84.42±1.8 µg/ml). In FRAP method, the highest antioxidant capacity was found in crude extract (5.4±0.3 mg/ml) and buthanol fractions (5.51±0.4 mg/ml), respectively. The highest amount of phenolic compounds was detected in ethyl acetate fraction of Myrtus communis (17.5±0.001 µg/g). The highest amount of flavonoids was found in crude extract of Myrtus communis (171.9±7.3 µg/ml). Overall, we can suggest that the leaves of Myrtus communis can be used as antioxidant and as a food additives to avoid oxidative degradation of foods.
- Gortzi O, Lalas S, Chinou I, Tsaknis J. Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. Eur Food Res Technol. 2008; 226: 583-90.
- Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr rev. 1998; 56: 317-33.
- Feibt C, Franke L, Appendino G, Werz O. Identification of molecular targets of the oligomeric nonprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds. J Pharmacol Exper Ther. 2005; 315: 389-96.
- Montoro P, Tuberosco IC, Piacente S, Perrone A, Defeo V, Cabras P, etal. Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. J Pharm Biomed Anal. 2006; 41: 1614-19.
- Pichon PN, Raynaud J, Debourchieu L, Josheph MJ. Sur la presence d'un heteroside acyle de la quercetine dans les feuilles de Myrtus communis, L.(Myrtaceae). Pharmazie. 1989; 44: 508-509.
- Mansouri S, Foromadi A, Ghaneie T, Najar AG . Antibacterial activity of the crude extracts and fractionated constituents of Myrtus communis. Pharm Biol. 2001; 39: 399-401.
- Shahidi Bonjar G. Antibacterial screening of plants used in Iranian folkloric medicine. Fitoterapia. 2004; 75: 231-5.
- Jayasinghe C, Goton N, Aoki T, Wada S. Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). J Agric Food Chem. 2003; 51: 4442-4449.
- Moein S, Moein MR, Khoshound MJ, Kalanteri T. In vitro antioxidant properties evaluation of 10 Iranian medicinal plants by different methods. Iran Red Crescent Med J. 2012; 14: 771.
- Sabahi Z, Zarshenas MM, Essential Oil Composition and in vitro Antioxidant Activity of Ethanolic Extract of Thymus daenensis Celak from Iran. Global J Pharmacol. 2013; 7: 153-58.
- Zhishen, J, Mengcheng, T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chem. 1999; 64: 555-9.
- Moein MR, Moein S, Ahmadizadeh S. Radical scavenging and reducing power of Salvia mirzayanii subfractions. Molecules. 2008; 13: 2804-13.
- Moein S, Farzami B, Khaghani S, Moein MR, larijani B. Antioxidant properties and prevention of cell cytotoxicity of Phlomis persica Boiss. Daru. 2007; 15: 83-8.