A reversed-phase high performance liquid chromatography approach for analysis of 5-Fluorouracil

Soha Azadi, Amir Azadi, Hajar Ashrafi, Soliman Mohammadi-Samani


A reproducible, sensitive, accurate and selective high performance liquid chromatographic (HPLC) method with ultraviolet absorbance detection has been developed and validated to quantitate 5-Fluorouracil (5-FU), an antimetabolite chemotherapeutic agent used in treatment of colorectal cancer as a drug of choice. Chromatographic separations were performed on a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) as the reversed stationary phase, eluted with a mobile phase composed of deionized water and methanol (95:5) with the flow rate of 1.0 mL/min. The UV wavelength was set at 261nm. The method produced linear responses throughout 5-fluorouracil range of 25-500 ng/ml with a correlation coefficient of 0.998. A limit of quantitation (LOQ) was established at 25 ng/ml. The within-day and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests indicate a proper separation efficacy as well as accuracy and sensivity of method which is a potential tool in many pharmaceutical studies such as drug delivery of this anti-cancer agent. 

Full Text:


DOI: http://dx.doi.org/10.1111%2Ftips.v3i1.119


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Contact Information:
  • Trends in Pharmaceutical Sciences, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR, Iran.
    Tel/Fax: +98 71 32424128 Ext: 297
  • P.O. Box: 17345-1583 Shiraz, Iran
  • Web site: tips.sums.ac.ir
    Email:tips@sums.ac.ir; irantips@gmail.com 
  • pISSN: 2423-3722   eISSN: 2423-5652