Multi-functional effects of curcumin on breast cancer cells

Document Type : Original Article

Authors

1 Student research committee, Birjand University of Medical Sciences, Birjand, the Islamic Republic of Iran

2 Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran

10.30476/tips.2024.101513.1230

Abstract

The rate at which cancer develops is significantly influenced by angiogenesis. The multifunctional effects of curcumin on breast cancer cells were examined in this study at the level of gene expression. Using the MTT assay, the cytotoxicity impact of curcumin was assessed. Real-time PCR was used to quantify the expression of a few genes involved in the angiogenesis, and apoptosis of MDA-231-MB cells. The results indicated that curcumin inhibited cancer proliferation in a dose- and time-dependent manner. The IC50 curcumin was about 40 µM and 15 µM after 48 h, and 96 h culture time respectively. Also, the quantitative analysis of gene expression showed that curcumin suppressed angiogenesis by decreasing the expression of VEGF and induced apoptosis by upregulation of Bax and down-regulation of Bcl-xl at the initial dose of toxicity concentration.

Highlights

Abolfazl Sadeghi (Google Scholar)

Mehdi Shakibaie (Google Scholar)

Keywords

References

1.    Jekabsons MB, Merrell M, Skubiz AG, Thornton N, Milasta S, Green D, Chen T, Wang YH, Avula B, Khan IA, Zhou YD. Breast cancer cells that preferentially metastasize to lung or bone are more glycolytic, synthesize serine at greater rates, and consume less ATP and NADPH than parent MDA-MB-231 cells. Cancer Metab. 2023 Feb 20;11(1):4. doi: 10.1186/s40170-023-00303-5. PMID: 36805760; PMCID: PMC9940388.
2.    Nishida N, Yano H, Nishida T, Kamura T, Kojiro M. Angiogenesis in cancer. Vasc Health Risk Manag. 2006;2(3):213-9. doi: 10.2147/vhrm.2006.2.3.213. PMID: 17326328; PMCID: PMC1993983.
3.    Chakraborty G, Jain S, Kale S, Raja R, Kumar S, Mishra R, Kundu GC. Curcumin suppresses breast tumor angiogenesis by abrogating osteopontin-induced VEGF expression. Mol Med Rep. 2008 Sep-Oct;1(5):641-6. doi: 10.3892/mmr_00000005. PMID: 21479462.
4.    Zare ME, Kansestani AN, Hemmati S, Mansouri K, Vaisi-Raygani A. The rate of aerobic glycolysis is a pivotal regulator of tumor progression. J Diabetes Metab Disord. 2021 Apr 1;20(1):523-531. doi: 10.1007/s40200-021-00774-7. PMID: 34178852; PMCID: PMC8212265.
5.    Peng B, Zhang SY, Chan KI, Zhong ZF, Wang YT. Novel Anti-Cancer Products Targeting AMPK: Natural Herbal Medicine against Breast Cancer. Molecules. 2023 Jan 11;28(2):740. doi: 10.3390/molecules28020740. PMID: 36677797; PMCID: PMC9863744.
6.    Siddiqui FA, Prakasam G, Chattopadhyay S, Rehman AU, Padder RA, Ansari MA, et al. Curcumin decreases Warburg effect in cancer cells by down-regulating pyruvate kinase M2 via mTOR-HIF1α inhibition. Sci Rep. 2018 May 29;8(1):8323. doi: 10.1038/s41598-018-25524-3. PMID: 29844464; PMCID: PMC5974195.
7.    Hu C, Li M, Guo T, Wang S, Huang W, Yang K, Liao Z, Wang J, Zhang F, Wang H. Anti-metastasis activity of curcumin against breast cancer via the inhibition of stem cell-like properties and EMT. Phytomedicine. 2019 May;58:152740. doi: 10.1016/j.phymed.2018.11.001. Epub 2018 Nov 12. PMID: 31005718.
8.    Guan F, Ding Y, Zhang Y, Zhou Y, Li M, Wang C. Curcumin Suppresses Proliferation and Migration of MDA-MB-231 Breast Cancer Cells through Autophagy-Dependent Akt Degradation. PLoS One. 2016 Jan 11;11(1):e0146553. doi: 10.1371/journal.pone.0146553. Retraction in: PLoS One. 2023 Mar 15;18(3):e0283354. PMID: 26752181; PMCID: PMC4708990.
9.    Guo W, Ding Y, Pu C, Wang Z, Deng W, Jin X. Curcumin inhibits pancreatic cancer cell proliferation by regulating Beclin1 expression and inhibiting the hypoxia-inducible factor-1α-mediated glycolytic pathway. J Gastrointest Oncol. 2022 Dec;13(6):3254-3262. doi: 10.21037/jgo-22-802. PMID: 36636058; PMCID: PMC9830349.
10.    Zhu Y, Bu S. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evid Based Complement Alternat Med. 2017;2017:5787218. doi: 10.1155/2017/5787218. Epub 2017 Sep 10. PMID: 29081818; PMCID: PMC5610853.
11.    Li P, Pu S, Lin C, He L, Zhao H, Yang C, et al. Curcumin selectively induces colon cancer cell apoptosis and S cell cycle arrest by regulates Rb/E2F/p53 pathway. J Mol Struct. 2022;1263:133180.
12.    Zhou Y, Kong Y, Jiang M, Kuang L, Wan J, Liu S, Zhang Q, Yu K, Li N, Le A, Zhang Z. Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines. Cancer Biol Ther. 2022 Dec 31;23(1):328-335. doi: 10.1080/15384047.2022.2058862. PMID: 35435150; PMCID: PMC9037542.
13.    Timoshenko A, Rastogi S, Lala P. Migration-promoting role of VEGF-C and VEGF-C binding receptors in human breast cancer cells. British journal of cancer. 2007;97(8):1090-8.
14.    Hu A, Huang JJ, Zhang JF, Dai WJ, Li RL, Lu ZY, et al. Curcumin induces G2/M cell cycle arrest and apoptosis of head and neck squamous cell carcinoma in vitro and in vivo through ATM/Chk2/p53-dependent pathway. Oncotarget. 2017 Apr 13;8(31):50747-50760. doi: 10.18632/oncotarget.17096. PMID: 28881600; PMCID: PMC5584201.
15.    Gabr SA, Elsaed WM, Eladl MA, El-Sherbiny M, Ebrahim HA, Asseri SM, Eltahir YAM, Elsherbiny N, Eldesoqui M. Curcumin Modulates Oxidative Stress, Fibrosis, and Apoptosis in Drug-Resistant Cancer Cell Lines. Life (Basel). 2022 Sep 13;12(9):1427. doi: 10.3390/life12091427. PMID: 36143462; PMCID: PMC9504331.
16.    Hu S, Xu Y, Meng L, Huang L, Sun H. Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells. Exp Ther Med. 2018 Aug;16(2):1266-1272. doi: 10.3892/etm.2018.6345. Epub 2018 Jun 22. PMID: 30116377; PMCID: PMC6090267.
Trends in Pharmaceutical Sciences
Volume 10, Issue 1
March 2024
Pages 51-56

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