Impact of two different extraction methods on chemical composition and antimicrobial activities of multi-ingredients essential oils and hydrosols

Fatemeh Ghavidel, Mohammad M. Zarshenas, Younes Ghasemi, Ahmad Gholami, Amirhossein Sakhteman, Pouya Faridi

Abstract


Herbal medicinal oils and hydrosols are of most useful preparations in folk Persian medicine. Till now no comprehensive evaluation has been performed on the impact of simultaneous extraction or mixing the extracted products on chemical composition profile of those preparations. Current study chemically assesses the impact of two different extraction methods for essential oils (EO) and hydrosols (separated and mixed) on chemical composition and activity. Samples of Mentha spicata L. (MS), Zataria multiflora Boiss. (ZM), Bunium persicum (Boiss.) B.Fedtsch. (BP) and Trachyspermum ammi (L.) Sprague (TA) were subjected to hydrodistillation, either individually or in combination with each other. Hydrosols and EO samples of each plant were mixed to prepared new hydrosol and poly-EO samples mixtures. All samples were injected to GC/MS for analysis. Moreover, anti-microbial activity of EOs and hydrosols were measured by MIC method. ATR-IR spectroscopies were used for recorded finger print from EOs. Carvone, Thymol, Cuminic aldehyde, Thymol were identified as the major constituents of MS, ZM, BP, TA EO samples, respectively. Hydrosol of MS, ZM, BP, and TA revealed to have Piperitenone, Carvacrol, Cuminol and Thymol as main components, respectively. The mixed oil samples, from first part had γ-Terpinene and Carvacrol as major components and hydrosol samples had Thymol as major component, respectively. In mixed oils and hydrosols the major components were γ-Terpinene and Thymol in respective order. This study showed that there were differences between main components, antimicrobial activity, antioxidant and ATR-IR spectroscopy of mixed samples in both preparation methods.


Keywords


Hydrosol, Essential oil, Traditional pharmacy, Traditional dosage form

Full Text:

PDF

References


Rezaeizadeh, H.; Alizadeh, M.; Naseri, M.; Ardakani, M. S., The Traditional Iranian Medicine Point of View on Health and. Iranian J Publ Health 2009, 38 (1), 169-172.

Zarshenas, M. M.; Petramfar, P.; Firoozabadi, A.; Moein, M. R.; Mohagheghzadeh, A., Types of headache and those remedies in traditional persian medicine. Pharmacognosy reviews 2013, 7 (13), 17-26.

Zargaran, A.; Zarshenas, M. M.; Hosseinkhani, A.; Mehdizadeh, A., Jawarish, a Persian traditional gastrointestinal dosage form. Pharmaceutical historian 2012, 42 (2), 24-5.

Rose, J., 375 Essential Oils and Hydrosols. Frog,Ltd.: Berkeley, California, 1999.

(a) Carson, C.; Riley, T., Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. Journal of applied bacteriology 1995, 78 (3), 264-269; (b) Ghasemi, Y.; Faridi, P.; Mehregan, I.; Mohagheghzadeh, A., Ferula gummosa fruits: an aromatic antimicrobial agent. Chemistry of natural compounds 2005, 41 (3), 311-314; (c) Abbaszadegan, A.; Dadolahi, S.; Gholami, A.; Moein, M.; Hamedani, S.; Ghasemi, Y.; Abbott, P., Antimicrobial and Cytotoxic Activity of Cinnamomum zeylanicum, Calcium Hydroxide, and Triple Antibiotic Paste as Root Canal Dressing Materials. The journal of contemporary dental practice 2015, 17 (2), 105-113; (d) Abbaszadegan, A.; Gholami, A.; Ghahramani, Y.; Ghareghan, R.; Ghareghan, M.; Kazemi, A.; Iraji, A.; Ghasemi, Y., Antimicrobial and cytotoxic activity of Cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. Iranian endodontic journal 2016, 11 (1), 44; (e) Nabavizadeh, M.; Abbaszadegan, A.; Gholami, A.; Sheikhiani, R.; Shokouhi, M.; Shams, M. S.; Ghasemi, Y., Chemical constituent and antimicrobial effect of essential oil from Myrtus communis leaves on microorganisms involved in persistent endodontic infection compared to two common endodontic irrigants: An in vitro study. Journal of conservative dentistry: JCD 2014, 17 (5), 449.

Sadraei, H.; Asghari, G.; Hajhashemi, V.; Kolagar, A.; Ebrahimi, M., Spasmolytic activity of essential oil and various extracts of Ferula gummosa Boiss. on ileum contractions. Phytomedicine 2001, 8 (5), 370-376.

(a) Hajhashemi, V.; Ghannadi, A.; Jafarabadi, H., Black cumin seed essential oil, as a potent analgesic and antiinflammatory drug. Phytotherapy Research 2004, 18 (3), 195-199; (b) Silva, J.; Abebe, W.; Sousa, S.; Duarte, V.; Machado, M.; Matos, F., Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. Journal of ethnopharmacology 2003, 89 (2), 277-283.

Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M., Biological effects of essential oils–a review. Food and chemical toxicology 2008, 46 (2), 446-475.

Ebrahimzadeh, H.; Yamini, Y.; Sefidkon, F.; Chaloosi, M.; Pourmortazavi, S. M., Chemical composition of the essential oil and supercritical CO 2 extracts of Zataria multiflora Boiss. Food chemistry 2003, 83 (3), 357-361.

(a) Edris, A. E., Identification and absolute quantification of the major water-soluble aroma components isolated from the hydrosols of some aromatic plants. Journal of Essential Oil Bearing Plants 2009, 12 (2), 155-161; (b) Verma, R. S.; Padalia, R. C.; Chauhan, A.; Singh, A.; Yadav, A. K., Volatile constituents of essential oil and rose water of damask rose (Rosa damascena Mill.) cultivars from North Indian hills. Natural product research 2011, 25 (17), 1577-1584.

(a) Suzane, C., Hydrosols: the next aromatherapy. Healing Arts Press: Rochester, Vermont, 2001; (b) Zarshenas, M. M.; Amirhossein, S.; Hamed, E.; Mahmoodreza, M., Trachyspermum ammi hydrosol, An almost pure source of thymol; Analysis of the recovered essential oil from different samples. International Journal of Pharmacognosy and Phytochemical Research 2015, 7 (3), 457-460.

Afsharypour S.; Shams Ardekani MR.; Mosaddegh M.; Ghannadi A.; Mohagheghzadeh, A.; Badr P., Introduction to iranian traditional pharmacy and pharmaceutical dosage forms. Choogan: Iran, Tehran, 1392.

In British Pharmacopoeia, Her Majesty’s stationary office: London, 1990.

Moein, M.; Zarshenas, M. M.; Delnavaz, S., Chemical composition analysis of rose water samples from Iran. Pharmaceutical biology 2014, 52 (10), 1358-1361.

Vahdani, M.; Faridi, P.; Zarshenas, M. M.; Javadpour, S.; Abolhassanzadeh, Z.; Moradi, N.; Bakzadeh, Z.; Karmostaji, A.; Mohagheghzadeh, A.; Ghasemi, Y., Major compounds and antimicrobial activity of essential oils from five Iranian endemic medicinal plants. Pharmacognosy Journal 2011, 3 (22), 48-53.

Adams, R., Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Corporation: Illinois, USA, 2007; Vol. 4th.

Sakhteman, A.; Keshavarz, R.; Mohagheghzadeh, A., ATR-IR fingerprinting as a powerful method for identification of traditional medicine samples: a report of 20 herbal patterns. Research Journal of Pharmacognosy 2015, 2 (3), 1-8.

Wold, S.; Esbensen, K.; Geladi, P., Principal component analysis. Chemometrics and intelligent laboratory systems 1987, 2 (1-3), 37-52.




DOI: http://dx.doi.org/10.1111%2Ftips.v4i3.187

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Contact Information:
  • Trends in Pharmaceutical Sciences, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR, Iran.
    Tel/Fax: +98 71 32424128 Ext: 297
  • P.O. Box: 17345-1583 Shiraz, Iran
  • Web site: tips.sums.ac.ir
    Email:tips@sums.ac.ir; irantips@gmail.com 
  • pISSN: 2423-3722   eISSN: 2423-5652