Multi-functional Anti-angiogenic and pro-apoptotic effects of curcumin on breast cancer cellseffects of curcumin on breast cancer cells

Document Type : Original Article

Authors

1 Student research committee, Birjand University of Medical Sciences, Birjand, the Islamic Republic of Iran

2 Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran

10.30476/tips.2024.101513.1230

Abstract

The rate at which cancer develops is significantly influenced by angiogenesis. The multifunctional effects of curcumin (CUR) on breast cancer cells were examined in this study at the level of gene expression. Using the MTT assay, the cytotoxicity impact of CUR was assessed. Real-time PCR was used to quantify the expression of a few genes involved in the angiogenesis, and apoptosis of MDA-231-MB cells. The results indicated that CUR inhibited cancer proliferation in a dose- and time-dependent manner. The IC50 CUR was about 20 µM and 10 µM after 48 h, and 96 h culture time respectively. Also, the quantitative analysis of gene expression showed that CUR suppressed angiogenesis by decreasing the expression of VEGF and induced apoptosis by upregulation of Bax and down-regulation of Bcl-xl at the initial dose of toxicity concentration. The expression ratio of Bax to Bcl-xl has increased by about 2 times compared to the control group.

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