%0 Journal Article %T Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method %J Trends in Pharmaceutical Sciences %I Shiraz University of Medical Sciences %Z 2423-3722 %A Mozafari, Negin %A Azadi, Soha %A Mohammadi Samani, Soliman %A Farjadian, Fatemeh %A Azadi, Amir %D 2019 %\ 03/01/2019 %V 5 %N 1 %P 41-46 %! Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method %K Citicoline %K Simvastatin %K HPLC %R 10.30476/tips.2019.81749.1006 %X To develop and evaluate a sensitive, accurate, rapid and reproducible high performance liquid chromatography analytical method for concurrent assay of simvastatin, a hyperlipidemia controlling agent, and citicoline, a psychostimulant agent, a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) used as a reversed stationary phase and mobile phase was water (previously adjusted with phosphoric acid to a pH of 5.5), methanol and acetonitrile (20:20:60) with the flow rate 1.0 ml/min. The ultraviolet detector was set at 247 nm. A linear correlation between each concentration and its own AUC within concentration ranges of 15 to 100 g/ml for citicoline and 7.5 to 50 g/ml for simvastatin with a correlation coefficient 0.9969 for citicoline and 0.994 for simvastatin were produced. The within and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests show an accurate, rapid and robust HPLC-UV method for successful analysis of both simvastatin and citicoline simultaneously. %U https://tips.sums.ac.ir/article_44989_257b890afc83bc99e5352ee938216eb9.pdf