TY - JOUR ID - 44989 TI - Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method JO - Trends in Pharmaceutical Sciences JA - TIPS LA - en SN - 2423-3722 AU - Mozafari, Negin AU - Azadi, Soha AU - Mohammadi Samani, Soliman AU - Farjadian, Fatemeh AU - Azadi, Amir AD - Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Department of Clinical Pharmacy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran AD - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Y1 - 2019 PY - 2019 VL - 5 IS - 1 SP - 41 EP - 46 KW - Citicoline KW - Simvastatin KW - HPLC DO - 10.30476/tips.2019.81749.1006 N2 - To develop and evaluate a sensitive, accurate, rapid and reproducible high performance liquid chromatography analytical method for concurrent assay of simvastatin, a hyperlipidemia controlling agent, and citicoline, a psychostimulant agent, a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) used as a reversed stationary phase and mobile phase was water (previously adjusted with phosphoric acid to a pH of 5.5), methanol and acetonitrile (20:20:60) with the flow rate 1.0 ml/min. The ultraviolet detector was set at 247 nm. A linear correlation between each concentration and its own AUC within concentration ranges of 15 to 100 g/ml for citicoline and 7.5 to 50 g/ml for simvastatin with a correlation coefficient 0.9969 for citicoline and 0.994 for simvastatin were produced. The within and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests show an accurate, rapid and robust HPLC-UV method for successful analysis of both simvastatin and citicoline simultaneously. UR - https://tips.sums.ac.ir/article_44989.html L1 - https://tips.sums.ac.ir/article_44989_257b890afc83bc99e5352ee938216eb9.pdf ER -