Evaluation of radiochemical purities of some radiopharmaceuticals in Shiraz Namazi teaching hospital

Document Type : Research(Original) Article


1 1- Department of medicinal chemistry, faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran 2- Nuclear Medicine and Molecular Imaging Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Department of nuclear medicine, Namazi hospital, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Department of medicinal chemistry, faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

4 1-Nuclear Medicine and Molecular Imaging Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2- Medical Radiation Engineering Department, Shahid Beheshti University, Tehran, Iran.

5 Department of medicinal chemistry, faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.


Radiopharmaceuticals are a special group of drugs since many of them are eventually prepared in the hospital and the nuclear medicine department is therfore responsible for meeting quality criteria such as sterility, radionuclide, radiochemical and chemical purity. In this study radiochemical purity for more than 300 preparations of three different radiopharmaceutical formulations from commercial kits were tested using instant thin layer chromatography. The formulations namely 99mTc-DTPA, 99mTc-MDP and 99mTc-MIBI were obtained from Pars Isotope Co. Several paper chromatographic systems were used in this study. The result showed that the most observed impurities were hydrolyzed reduced 99m technetium.  After the preliminary study we decided to change our imaging schedule in the nuclear medicine department and the sequence of imaging was changed.Cosequently, the results showed better radiochemical purities. Our equipments for detetion of radioactivity in paper chromatography were changed, for example the old dose calibrator was replaced by a gamma camera or a more accurate dose calibrator. Also, the thin layer chromatography systems  were changed to those recommended by the factory  and the new results showed much better radiochemical purities.

  1. Zimmerman BE, Herbst C, Norenberg JP, Woods MJ. International guidance on the establishment of quality assurance programmes for radioactivity measurement in nuclear medicine. Applied Radiation and Isotopes. 2006 10//;64(10–11):1142-6.
  2. Chattopadhyay S, Saha Das S, Chandra S, De K, Mishra M, Ranjan Sarkar B, et al. Synthesis and evaluation of< sup> 99m Tc-moxifloxacin, a potential infection specific imaging agent. Applied Radiation and Isotopes. 2010;68(2):314-6.
  3. Roohi S, Mushtaq A, Jehangir M, Malik S. Synthesis, quality control and biodistribution of 99m Tc-Kanamycin. Journal of radioanalytical and nuclear chemistry. 2006;267(3):561-6.
  4. Spies H, Pietzsch H-J. Stannous chloride in the preparation of 99mTc pharmaceuticals. Technetium-99m Pharmaceuticals: Springer; 2007. p. 59-66.
  5. Zolle I. Technetium-99m pharmaceuticals Springer; 2007.
  6. Dzik-Jurasz A. Molecular imaging in vivo: an introduction. British journal of radiology. 2003;76(suppl 2):S98-S109.
  7. Chu T, Zhang Y, Liu X, Wang Y, Hu S, Wang X. Synthesis and biodistribution of< sup> 99m Tc-carbonyltechnetium-labeled fatty acids. Applied radiation and isotopes. 2004;60(6):845-50.
  8. Jurisson S, Berning D, Jia W, Ma D. Coordination compounds in nuclear medicine. Chemical Reviews. 1993;93(3):1137-56.
  9. Tilden RL, Jackson J, Enneking WF, Deland FH, McVey JT. 99mTc-polyphosphate: Histological localization in human femurs by autoradiography. Journal of Nuclear Medicine. 1973;14(8):576-8.
  10. Zolle I. Technetium-99m pharmaceuticals: preparation and quality control in nuclear medicine: Springer; 2007.
  11. Thakur M, DeFulvio J, Richard M, Park C. Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. International journal of radiation applications and instrumentation Part B Nuclear medicine and biology. 1991;18(2):227-33.
  12. Alberto R, Schibli R, Egli A, Schubiger AP, Abram U, Kaden TA. A novel organometallic aqua complex of technetium for the labeling of biomolecules: synthesis of [99mTc (OH2) 3 (CO) 3]+ from [99mTcO4]-in aqueous solution and its reaction with a bifunctional ligand. Journal of the American Chemical Society. 1998;120(31):7987-8.
  13. Stelmach HA, Quinn Iii JL. Radiopharmaceutical quality control. Seminars in Nuclear Medicine. 1974 7//;4(3):295-303.
  14. http://www.parsisotope.com/en/pdf/general.pdf.