Chemical compositions of two endemic Thymus species essential oil

Document Type : Research(Original) Article


1 Department of Chemistry, Yasouj University, Yasouj, Iran

2 Medical Plants Processing Research Center and Department of Pharmacognosy School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Agriculture, Yasouj University, Yasouj, Iran

4 Central Research Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


Thymus is one of the most important members of Lamiaceae family. Aerial parts of the plant have been widely used in medicine. It has been reported that most of these effects are related to phenolic compounds especially thymol and carvacrol in Thymus essential oil.In this study, aerial parts of Thymus daenensis and Thymus lancifolius were collected from Kohgiluyeh and Boyer-Ahmad. Essential oil of aerial parts of these plants was gained by hydrodistillation method and the chemical compositions were analyzed by gas chromatography/ Mass spectrometry (GC/MS). The major components of the essential oil of Thymus daenensis were thymol (39.91%), carvacrol (29.93%), linalool (5.55%), caryophyllene (3.5%) and geraniol (3.09%), whereas the major components of the essential oil of Thymus lancifolius were: carvacrol (25.55%), thymol (20.79%), linalool (16.8%), α- terpineol(6.34%), borneol (4.00%), caryophyllene (3.98%), p-cymene (3.38%) and cis-linalool oxide (3.21%). Linalool was reported as another major component in Thymus lancifolius.

  1. Rechinger KH. Flora Iranica. Austria: Graz: Akademische Druck u. Verlagsanstalt; 1982.
  2. Inouye S, Takizawa T, Yamaguchi H. Antibacterial effects of essential oil and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrob Chemother. 2001; 47: 565-73.
  3. Plaza P, Torres R, Usall J, Lamarca N, Viasa I. Evalauiton of the potential of commercial post–harvest application of essential oils to control citrus decay. J Hort Sci Biotech. 2004; 79: 935-40.
  4. Cherallier A. The Encyclopedia of Medicinal Plants. London: Dorling Kindersley Limited; 1996.
  5. Ghasemipirbalouti A. Medicinal plants used in Chaharmahal and Bakhtyari districts of Iran. Herba Pol. 2009; 55: 69-75.
  6. Rustaiyan A, Lajevardi T, Rabbani M, Yari M, Masoudi S. Chemical constituents of the essential oil of Thymus kotschyanus Boiss. and Hohen. From Iran. Daru. 1999; 7: 27-8.
  7. Tümen G, KirimerN, BaŜer. Composition of the essential oils of Thymus species growing in Turkey. Chem Nat Compd. 1995; 31: 42-7.
  8. Miceli A, Negro C, Tommasi L. Essential oil variability in Thymbra capitata (L.) Cav. growing wild in Southern Apulia Italy. Biochem Syst Ecol. 2006; 34: 528-35.
  9. Massada Y. Analysis of essential oil by gas chromatography and spectrometry. New York: Wiley Press, 1976.
  10. Adams RP. Identification of essential oil components by gas chromatography quadrupole mass spectroscopy. Illinois: Allured Publishing, 1995.
  11. Sajjadi SE, Khatamsaz M. Composition of the essential oil of Thymus daenensis Celak. ssp. lancifolius (Celak.) Jalas. J Essent Oil Res. 2003; 15: 34–5.
  12. Jamzad Z. Flora of Iran. Lamiaceae Family. Iran: Tehran: Research Institute of Forests and Rangelands; 2013.
  13. Nickavar B, Mojab F, Dolat-Abadi R. Analysis of the essential oil of two Thymus species from Iran. Food Chem. 2005; 90: 609-11.
  14. Sabahi Z, Zarshenas MM, Farmani F, Faridi P, Moein S, Moein MR. Essential oil composition and in vitro antioxidant activity of ethanolic extract of Thymus daenensis Celak from Iran. Global J. Pharmacol. 2013; 7: 153-8.
  15. Alavi L, Barzegar M, Jabbari A, Naghdi Badi H. Effect of heat treatment on chemical composition and antioxidant property of thymus daenensis essential oil. J Med Plants. 2010; 9: 129-38.