Free radical scavenging activity and lipid peroxidation inhibition of Hypericum helianthemoides (spach) Boiss

Document Type : Research(Original) Article



Antioxidants are compounds that obstruct the oxidation of macromolecules in the body. In general, there are two categories of antioxidants, natural and synthetic. Recently, interest has been increased considerably for obtaining new natural antioxidants. In this study, the scavenging of free radicals such as DPPH, NO and OH by Hypericum helianthemoides extract was evaluated. Also, the antioxidant properties of this extract were evaluated by FRAP, FTC methods and determination phenolic compounds. The plant was collected from north of Fars Province and plant extraction was obtained using ethanol. In DPPH radical scavenging, different concentrations of the Hypericum extract were added to DPPH radical. In hydroxyl radical scavenging, Fenton reaction mixture, TCA and TBA were mixed with Hypericum extract. In nitric radical scavenging, nitropruside was mixed with Hypericum extract and then sulphanilic acid, naphthylene diamine were added. In determination of phenolic compounds, Folin-ciocalteu and sodium carbonate were added to Hypericum extract. In DPPH radical scavenging, the IC50 of Hypericum extract (309.35±6.5µg/ml) was higher than the antioxidant standards, BHT (IC50=81.9±2.6 µg/ml) and quercetin (IC50=60.04±6.48 µg/ml). The highest scavenging of hydroxyl radicals was observed in Hypericum extract (70.3±0.8%, 125 µg/ml). In gallic acid it was (73.8±3.3%). In 200 µg/ml of Hypericum extract scavenged NO radical (85.2±2.7%). In FRAP method, the IC50 of this extract was 109.7±10.5 µg/ml. In FTC method, the inhibition of lipid peroxidation by Hypericum extract, BHT and ascorbic acid were 59.2±2.2, 66.9±0.15, 64.06±0.02 respectively. Total phenol of the plant extract was 3±0.4 mg/g.//

  1. Narayanan R, Chandrasekaran S, Sooriamuthu S, Jerrine J, Nanjian R. Antioxidant activities and phytochemical analysis of methanol extract of leaves of Hypericum hookerianum. IJPPS. 2014;6:456-60.
  2. Velioglu Y, Mazza G, Gao L, Oomah B.Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998;46:4113-17.
  3. Apak R, Cuclu K, Demirata B, Qzyurek M, Celik SE, Bektasoglu, et al. Comparative evaluation of various total antioxidant assays applied to phenolic compounds with the CUPRAC Assay. Molecules. 2007;12:496-547.
  4. Moein S, Moein MR, Khoshnoud MJ, Kalantari, T. In vitro antioxidant properties
  5. evaluation of 10 Iranian medicinal plants by different methods. Iran Red Crescent Med J. 2012;14:771-5.
  6. Mockute D, Bernotiene G. The main citral geraniol and carvacrol chemotypes of the essential oil of Thymus pulegioides L. growing wild in Vilnius district (Lithuania). J Agric Food Chem. 1999;47:3787-90.
  7. Sabahi Z, Zarshenas MM, Farmani F, Faridi F, Moein S, Moein MR. Essential oil composition and in vitro antioxidant activity of ethanolic extract of Thymus daenensis celak from Iran. Global J Pharmacol. 2013;7:153-8.
  8. Olabinri BM, Odedire OO, Olaleye MT, adekunle AS, Ehigie LO, Olabinri PF. In vitro evaluation of hydroxyl and Nitric oxide radical scavenging activities of Artemether. Res J Biol Sci. 2010;5:102-5.
  9. DeMartino L, Bruno M, Fromisano C, Feo V De, Napolitane F, Rossellis S. Chemical composition and antimicrobial activity of the essential oils from two species of Thymus growing wild in southern Italy. Molecules. 2009;14: 6414-24.
  10. Moein S, Moein MR. Antioxidant activities and phenolic content of Juniperus excels extract. Iranian J Pharm Sci. 2010;6:133-40.
  11. Moein MR, Moein S, Ahmadizadeh
  12. S. Radical scavenging and reducing power
  13. Salvia mirzayanii subfractions. Molecules. 2008;13:2804-13.
  14. Ozturk N, Tuncel M, Potoglu-Erkara I. Phenolic compounds and antioxidant activities of some Hypericum species: A comparative study with Hypericum. Pharm Biol. 2009;47:120-7.