Production of a novel multi-epitope vaccine based on outer membrane proteins of Klebsiella pneumoniae

Document Type : Research(Original) Article

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Abstract

Klebsiella pneumoniae is a hospital-acquired pathogen that leads to various infections. Hence, efforts to develop an effective vaccine against that pathogen are well documented. Our interest is the production of the previously designed multi-epitope vaccine construct against the K. pneumoniae in a prokaryotic host. Therefore, a new construct containing the nucleotide sequence of the novel vaccine was successfully expressed in Escherichia coli and then purified by Ni-NTA spin column. The purified recombinant protein can be considered as potential vaccine candidate for wet-laboratory analysis aiming to fight K pneumoniae .

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References

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Trends in Pharmaceutical Sciences
Volume 1, Issue 3 - Serial Number 3
5
September 2015
Pages 167-172

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