Trends in Pharmaceutical Sciences and Technologies

Trends in Pharmaceutical Sciences and Technologies

Antidiabetic and Synergistic Effects Study of Anthocyanin Fraction from Berberis integerrima Fruit on Streptozotocin-Induced Diabetic Rats Model

Document Type : Research(Original) Article

Authors
Zahra Sabahi 1
Mohammad Javad Khoshnood-Mansoorkhani 2
Solmaz Rahmani Namadi 2
Mahmoodreza Moein 3
1 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
2 Department of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Introduction: Diabetes mellitus is a complex endocrine disorder. There is serious attempt to realize antidiabetic compound from natural sources to use with other drugs for reduction of diabetes complication.  Materials and Methods: Present study is based on the investigation of antihyperglycemic effect of anthocyanin fraction of Berberis integerrima (AFBI) fruits on some physiological parameters (glucose level, glycogen content and body weight) in normal and streptozotocin-induced (STZ-induced) diabetic rats and evaluation of synergic effect of this fraction with metformin and glybenclamid. Male Sprague dawley rat were divided into nine groups: healthy control group, diabetic control group, diabetic groups treated with anthocyanin fraction (200, 400 and 1000 mg/kg, respectively); diabetic groups treated with glybenclamide and metformin separately, diabetic groups treated with glybenclamide + anthocyanin fraction (1000 mg/kg), metformin +anthocyanin fraction (1000 mg/kg).  Results: Treatment of diabetic rats with AFBI (400, 1000mg/kg) significantly decreased blood glucose as compared with control. Moreover AFBI (400, 1000mg/kg) significantly increased liver glycogen and body weight in compared to control. Nevertheless there were no synergistic effects between anthocyanin fraction and metformin or glibenclamid on blood glucose, liver glycogen and body weight. Conclusion: The results of this study indicate that AFBI possesses hypoglycemic effects and may be considered for evaluation in future diabetes clinical studies.
Keywords
Berberis Integerrima
Antidiabetic
Synergistic Effects
Streptozotocin
  1. Rani AJ, Mythili SV. Study on Total Antioxidant Status in Relation to Oxidative Stress in Type 2 Diabetes Mellitus. J Clin Diagn Res. 2014;8(3):108-10.
  2. Brownlee M. The Pathobiology of Diabetic Complications: A Unifying Mechanism. Diabetes. 2005; 1;54(6):1615-25.
  3. Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: A review. J Biochem Mol Toxicol. 2003;17(1):24-38.
  4. Ashraf H, Zare S. Preventive Effects of Aqueous Extract of Berberis integerrima Bge. Root on Liver Injury Induced by Diabetes Mellitus (Type 1) in Rats. Iran J Pharm Res. 2015;14(1):335-43.
  5. Lila MA. Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol. 2004;2004 (5):306-13.
  6. Laleh GH, Frydoonfar H, Heidary R, Jameei R, Zare S. The effect of light, temperature, pH and species on stability of anthocyanin pigments in four Berberis species. Pak J N. 2006;5(1):90-2.
  7. Rodriguez-Saona LE, Giusti MM, Durst RW, Wrolstad RE. Development and process optimization of red radish concentrate extract as potential natural red colorant. J Food Process Pres. 2001;25(3):165-82.
  8. Butler NA, Lee EYC, Whelan WJ. A protein-bound glycogen component of rat liver. Carbohyd Res. 1977;55(1):73-82.
  9. Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi S, Farhangi A, Verdi AA, et al. Induction of diabetes by Streptozotocin in rats. Indian J Clin Biochem. 2007; 22(2):60-4.
  10. Johansen JS, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical practice. Cardiovasc Diabetol. 2005;4(5).
  11. Ashraf H, Heidari R, Nejati V. Antihyperglycemic and Antihyperlipidemic Effects of Fruit Aqueous Extract of Berberis integerrima Bge. in Streptozotocin-induced Diabetic Rats. Iran J Pharm Res. 2014;13(4):1313-8.
  12. Garzon GA, Wrolstad RE. Major anthocyanins and antioxidant activity of Nasturtium flowers (Tropaeolum majus). Food Chem. 2009;114(1):44-9.
  13. Fan-Chiang H-J, Wrolstad RE. Anthocyanin Pigment Composition of Blackberries. J Food Sci. 2005;70(3):C198-C202.
  14. Ngo T, Wrolstad RE, Zhao Y. Color Quality of Oregon Strawberries—Impact of Genotype, Composition, and Processing. J Food Sci. 2007;72(1):C25-C32.
  15. Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancrea. Physiol Res. 2001;50(6):537-46.
  16. Grace MH, Ribnicky DM, Kuhn P, Poulev A, Logendra S, Yousef GG, et al. Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, Vaccinium angustifolium Aiton. Phytomedicine. 2009;16(5):406-15.
  17. Ghosh D, Konishi T. Anthocyanins and anthocyanin-rich extracts: role in diabetes and eye function.Asia Pac J Clin Nutr. 2007; 16(2):200-8.
  18. Huang B, Wang Z, Park JH, Ryu OH, Choi MK, Lee J-Y, et al. Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Nutr Res Pract. 2015;9(1):22-9.
  19. Jayaprakasam B, Vareed SK, Olson LK, Nair MG. Insulin Secretion by Bioactive Anthocyanins and Anthocyanidins Present in Fruits. J AgrFood Chem. 2005;53(1):28-31.
  20. Kong J-M, Chia L-S, Goh N-K, Chia T-F, Brouillard R. Analysis and biological activities of anthocyanins. Phytochemistry. 2003;64:923-33.
  21. Yassin K, Huyen VTT, Hoa KN, Östenson CG. Herbal Extract of Gynostemma Pentaphyllum Decreases Hepatic Glucose Output in Type 2 Diabetic Goto-Kakizaki Rats. Int J Biomed Sci. 2011;7(2):131-6.
  22. Rao PV, Madhavi K, Naidu MD, Gan SH. Rhinacanthus nasutus Improves the Levels of Liver Carbohydrate, Protein, Glycogen, and Liver Markers in Streptozotocin-Induced Diabetic Rats. Evid Based Complement Alternat Med. 2013; Article ID 102901,7.
  23. Tsuda T, Horio F, Uchida K, Aoki H, Osawa T. Dietary Cyanidin 3-O-β-d-Glucoside-Rich Purple Corn Color Prevents Obesity and Ameliorates Hyperglycemia in Mice. J Nutr. 2003;133(7):2125-30.
  24. Tsuda T, Ueno Y, Aoki H, Koda T, Horio F, Takahashi N, et al. Anthocyanin enhances adipocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes. Biochem Bioph Res Co. 2004; 316(1):149-57.
  25. Tsuda T, Ueno Y, Yoshikawa T, Kojo H, Osawa T. Microarray profiling of gene expression in human adipocytes in response to anthocyanins. Biochem Pharmacol. 2006;71(8):1184–97.