Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method

Mozafari, Negin; Azadi, Soha; Mohammadi Samani, Soliman; Farjadian, Fatemeh; Azadi, Amir

doi:10.30476/tips.2019.81749.1006

Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method

Document Type : Research(Original) Article

Authors

Negin Mozafari ¹

Soha Azadi ²

Soliman Mohammadi Samani ¹

Fatemeh Farjadian ³

Amir Azadi ¹

¹ Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

² Department of Clinical Pharmacy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

³ Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

10.30476/tips.2019.81749.1006

Abstract

To develop and evaluate a sensitive, accurate, rapid and reproducible high performance liquid chromatography analytical method for concurrent assay of simvastatin, a hyperlipidemia controlling agent, and citicoline, a psychostimulant agent, a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) used as a reversed stationary phase and mobile phase was water (previously adjusted with phosphoric acid to a pH of 5.5), methanol and acetonitrile (20:20:60) with the flow rate 1.0 ml/min. The ultraviolet detector was set at 247 nm. A linear correlation between each concentration and its own AUC within concentration ranges of 15 to 100 g/ml for citicoline and 7.5 to 50 g/ml for simvastatin with a correlation coefficient 0.9969 for citicoline and 0.994 for simvastatin were produced. The within and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests show an accurate, rapid and robust HPLC-UV method for successful analysis of both simvastatin and citicoline simultaneously.

Keywords

Citicoline

Simvastatin

HPLC