Effects of Sildenafil and Vitamin E on Paraquat-Induced Pulmonary Fibrosis in Rats.

Siahpour, Fouziyeh; Sadeghi, Hosein; Panahi Kokhdan, Esmaeel; Khoshnoud, Mohammad Javad

doi:10.30476/tips.2024.103997.1255

Effects of Sildenafil and Vitamin E on Paraquat-Induced Pulmonary Fibrosis in Rats.

Document Type : Original Article

Authors

¹ Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

² Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

10.30476/tips.2024.103997.1255

Abstract

Background & Objectives: Paraquat, which is specifically concentrated in lung tissue, leads to lung damage through oxidative and inflammatory processes. Pulmonary fibrosis occurs due to an imbalance in the antioxidant system and inflammation. Drugs like sildenafil, which inhibit phosphodiesterase enzymes, are used to treat pulmonary arterial hypertension. Antioxidants such as vitamin E can restore the oxidant-antioxidant balance. This study aimed to evaluate the effects of sildenafil and vitamin E on paraquat-induced pulmonary fibrosis.

Methods: Male Wistar rats were used in animal studies. Paraquat was intratracheally injected at a single dose of 40 mg/kg to induce lung fibrosis. Three groups were studied: Sildenafil administered orally (10 mg/kg) for 14 days. Vitamin E administered orally (500 mg/kg) for 14 days. Simultaneous administration of vitamin E (500 mg/kg) and sildenafil (10 mg/kg) for 14 days. Biochemical and histopathological tests were performed and compared with a control group.

Results: Paraquat administration caused fibrosis and an oxidant-antioxidant imbalance in lung tissue. Treatment with sildenafil and vitamin E increased glutathione (GSH) and superoxide dismutase (SOD) levels while decreasing malondialdehyde (MDA) and hydroxyproline (HYP) levels compared to the paraquat group. Tumor Necrosis Factor-α (TNF-α) showed no significant changes in the study group compared to the control group.

Conclusion: Treatment with sildenafil and vitamin E improved the oxidant-antioxidant balance in lung tissue exposed to paraquat. Additionally, this study demonstrated significant antioxidant effects of sildenafil and vitamin E against paraquat-induced oxidative damage.

Keywords