Membrane Stabilization and Inhibition of Protein Denaturation as Mechanisms of the Anti-Inflammatory Activity of some Plant Species

Document Type : Original Article

Authors

1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria

2 Department of Science Laboratory Technology, Faculty of Pure and Applied Science, Osun State College of Technology, Esa-oke, Nigeria.

3 Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University,Ile-Ife, Nigeria.

Abstract

Several reports have shown the potential of medicinal plants as vital sources of anti-inflammatory agents and this study is aimed at investigating the anti-inflammatory potential of selected medicinal plants used for the treatment of inflammation in folk medicine.
The methanol and acetone extract of Lecaniodiscus cupanioides, Talinum fruticosum, Ocimum gratissimum, Senna occidentalis, Senna alata were subjected to anti-inflammatory assays using membrane stability and inhibition of albumin denaturation methods and absorbance measured at 560 and 660 nm respectively. The mean IC50 and SEM values were determined using normalized response variable through non-linear regression XY analysis on prism graphpad® (7.0). In the membrane stability experiment, the extracts tested showed varying levels of erythrocyte lysis inhibition within the range of (IC50: 89.08 ± 6.339 µg/mL≤IC50≤278.3 ± 5.678 µg/mL) while the standard drug (ibuprofen) has an IC50: 61.93 ± 8.359 µg/mL, Lecaniodiscus cupanioides acetone (IC50 of 89.08 ± 6.339 µg/mL) extract showed to be most active when compared to other extracts. In the inhibition of albumin denaturation experiment, the tested extracts showed different level of activity within the range of (IC50:22.89±5.52 µg/mL≤IC50≤210.6±6.71 µg/mL). The acetone extracts of senna occidentalis (22.89 ± 5.52 µg/mL) and Lecaniodiscus cupanioides (80.9 ± 9.11 µg/mL) showed significant activity with the lowest IC50 compared to other extracts while the standard drug (aspirin) had an IC50 of (4.055±2.72 µg/mL).
The findings of this study shows that Lecaniodiscus cupanioides acetone extract has the most significant anti-inflammatory properties, therefore justifying it's traditional use in the treatment of inflammatory injury and tissue damage.

Keywords

References

1. Iwalewa EO, McGaw LJ, Naidoo V, Eloff JN. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. Afr J Biotechnol. 2007;6:2868-2885. doi: 10.5897/AJB2007.000-2457
2. Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004 Nov;96(3):229-45. doi: 10.1254/jphs.crj04003x. Epub 2004 Nov 12. PMID: 15539763.
3. Newman DJ, Cragg GM. Advanced preclinical and clinical trials of natural products and related compounds from marine sources. Curr Med Chem. 2004 Jul;11(13):1693-713. doi: 10.2174/0929867043364982. PMID: 15279577.
4. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol. 1968 Mar;20(3):169-73. doi: 10.1111/j.2042-7158.1968.tb09718.x. PMID: 4385045.
5. Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, Sipeolu FO. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. Int J Plant Physiol Biochem. 2010;2(4):46-51.
6. Nafiu MO, Abdulsalam TA, Akanji MA. Phytochemical Analysis and Antimalarial Activity Aqueous Extract of Lecaniodiscus cupanioides Root. J Trop Med. 2013;2013:605393. doi: 10.1155/2013/605393. Epub 2013 Aug 1. PMID: 23983718; PMCID: PMC3747395.
7. Gbadamosi IT, Oloyede AA. The mineral, proximate and phytochemical components of ten Nigerian medicinal plants used in the management of arthritis. Afr J Pharm Pharmacol. 2014;8:638-643. DOI: 10.5897/AJPP
8. Agnel Arul John N, Shobana, G. Anti-inflammatory activity of Talinum fruticosum L. on formalin induced paw oedema in albino rats. J App Pharm Sci. 2012;2:123-127.
9. Ueda-Nakamura T, Mendonça-Filho RR, Morgado-Díaz JA, Korehisa Maza P, Prado Dias Filho B, Aparício Garcia Cortez D, et al. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int. 2006 Jun;55(2):99-105. doi: 10.1016/j.parint.2005.10.006. Epub 2005 Dec 15. PMID: 16343984.
10. Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complement Altern Med. 2005 Mar 11;5:6. doi: 10.1186/1472-6882-5-6. PMID: 15762997; PMCID: PMC1079793.
11. Chinenye A-O, Emmanuel H, Hassan L.G, Awojide K.O. Phytochemical Screening and Evaluation of Anti-Inflammatory Activity Studies of Ethyl Acetate Leaves Extract from Ocimum gratissimum (L.). J Dent Med Sci. 2016;15:16-22.
12. Aguiyi JC, Obi CI, Gang SS, Igweh AC. Hypoglycaemic activity of Ocimum gratissimum in rats. Fitoterapia. 2000 Aug;71(4):444-6. doi: 10.1016/s0367-326x(00)00143-x. PMID: 10925022.
13. Ghani A. Medicinal Plants of Bangladesh with chemical constituents and uses. 2nd edn, Asiatic Society of Bangladesh. 2003.
14. Fernand VE, Dinh DT, Washington SJ, Fakayode SO, Losso JN, van Ravenswaay RO, et al. Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography. Talanta. 2008 Jan 15;74(4):896-902. doi: 10.1016/j.talanta.2007.07.033. Epub 2007 Aug 8. PMID: 18371725; PMCID: PMC2276639.
15. Akinmoladun AC, Obuotor EM, Farombi EO. Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. J Med Food. 2010 Apr;13(2):444-51. doi: 10.1089/jmf.2008.0292. PMID: 20192848.
16. Khan MR, Kihara M, Omoloso AD. Antimicrobial activity of Cassia alata. Fitoterapia. 2001 Jun;72(5):561-4. doi: 10.1016/s0367-326x(00)00335-x. PMID: 11429256.
17. Manach C, Williamson G, Morand C, Scalbert A, Rémésy C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 2005 Jan;81(1 Suppl):230S-242S. doi: 10.1093/ajcn/81.1.230S. PMID: 15640486.
18. Somchit MN, Reezal I, Nur IE, Mutalib AR. In vitro antimicrobial activity of ethanol and water extracts of Cassia alata. J Ethnopharmacol. 2003 Jan;84(1):1-4. doi: 10.1016/s0378-8741(02)00146-0. PMID: 12499068.
19. Lewis A, Levy A. Anti-inflammatory activities of Cassia alata leaf extract in complete Freund's adjuvant arthritis in rats. West Indian Med J. 2011 Dec;60(6):615-21. PMID: 22512217.
20. Sakat S, Juvekar AR, Gambhire MN. In-vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Sci. 2010;2:146-156. doi:10.1055/s-0029-1234983
21. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in-vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed. 2012;2(1):S178-S180. https://doi.org/10.1016/S2221-1691(12)60154-3
22. Agrawal SS, Paridhavi M. Herbal drug technology. Edn 2, University press Pvt. Ltd. Hyderabad, 2007.
23. Ferrali M, Signorini C, Ciccoli L, Comporti M. Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, divicine and isouramil. Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):295-301. doi: 10.1042/bj2850295. PMID: 1637315; PMCID: PMC1132780.
24. Mounnissamy VM, Kavimani S, Balu V, Drlin QS. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanol extract of Canjera rehedi. Iran J Pharmacol Ther. 2008; 6:235-237.
25. Akinpelu BA, Makinde AM, Isa MO, Taiwo OP, Ojelabi, OM & Oyedapo OO. In-vitro Evaluation of Membrane Stabilizing Potential of Selected Bryophyte Species. Eur J Med Plants. 2015;6(3):181-190 doi.org/10.9734/EJMP/2015/7629.
26. Aitdafoun M, Mounier C, Heymans F, Binisti C, Bon C, Godfroid JJ. 4-Alkoxybenzamidines as new potent phospholipase A2 inhibitors. Biochem Pharmacol. 1996 Mar 22;51(6):737-42. doi: 10.1016/0006-2952(95)02172-8. PMID: 8602868.
27. Sadique J, Chandra T, Thenmozhi V, Elango V. Biochemical modes of action of Cassia occidentalis and Cardiospermum halicacabum in inflammation. J Ethnopharmacol. 1987 Mar-Apr;19(2):201-12. doi: 10.1016/0378-8741(87)90042-0. PMID: 3613609.
28. Kumar V, Bhat ZA, Kumar D, Khan NA, Chashoo IA. Evaluation of anti-inflammatory potential of leaf extracts of Skimmia anquetilia. Asian Pac J Trop Biomed. 2012 Aug;2(8):627-30. doi: 10.1016/S2221-1691(12)60109-9. PMID: 23569983; PMCID: PMC3609364.
29. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils--a review. Food Chem Toxicol. 2008 Feb;46(2):446-75. doi: 10.1016/j.fct.2007.09.106. Epub 2007 Sep 29. PMID: 17996351.
30. Owoyele VB, Oloriegbe YY, Balogun EA, Soladoye AO. Analgesic and anti-inflammatory properties of Nelsonia canescens leaf extract. J Ethnopharmacol. 2005 May 13;99(1):153-6. doi: 10.1016/j.jep.2005.02.003. PMID: 15848036.
31. Metowogo K, Agbonon A, Eklu-Gadegbeku K, Aklikokou AK, Gbeassor M. Anti-ulcer and anti-inflammatory effects of hydr-alcohol extract of Aloe buettneri A. Berger (Lilliaceae). Trop J Pharmaceut Res. 2008;7(1):907-912. DOI: 10.4314/tjpr.v7i1.14676
32. Kalyanpur SG, Pohujani S, Nack SR, Seth UK. Study of biochemical effects of anti-inflammatory drugs in carrageenin-induced oedema and cotton pellet granuloma. Biochem Pharmacol. 1968; 17, 797-803. doi: 10.1016/0006-2952(68)90016-6
33. Govindappa M, Naga SS, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in-vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L). Hitch. J Pharmacol Phytother. 2011;3(3):43-51. https://doi.org/10.5897/JPP.9000012
34. Raju GS, Moghal MM, Hossain MS, Hassan MM, Billah MM, Ahamed SK, et al. Assessment of pharmacological activities of two medicinal plant of Bangladesh: Launaea sarmentosa and Aegialitis rotundifolia roxb in the management of pain, pyrexia and inflammation. Biol Res. 2014 Oct 28;47(1):55. doi: 10.1186/0717-6287-47-55. PMID: 25418519; PMCID: PMC4416252.
35. Yemitan OK, Adeyemi OO. CNS depressant activity of Lecaniodiscus cupanioides. Fitoterapia. 2005 Jul;76(5):412-8. doi: 10.1016/j.fitote.2005.02.010. PMID: 15955638.
36. Yemitan OK, Adeyemi OO, Adeogun OO. Analgesic activities of the aqueous root extract Lecaniodiscus cupanioides. West Afr J Pharmacol Drug Res. 2005;20:10-14. doi: 10.4314/wajpdr.v20i1.14738.
37. Akinwunmi KF, Oyedapo OO. In-vitro Anti-inflammatory Evaluation of African Nutmeg (Monodora myristica) Seeds.Eur J Med Plants. 2015;8(3):167-174. doi:10.9734/EJMP/2015/17853
Trends in Pharmaceutical Sciences
Volume 7, Issue 4
December 2021
Pages 269-278

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