Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake

Document Type : Research(Original) Article

Authors

1 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

2 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Urmia Lake is one of the largest hypersaline lakes in the world. Water evaporation and saturation cause a lot of salt crystals formation on the lake beach. In this study, extremely halophilic strains were isolated from salt crystals that formed in distinct regions of lake. The isolation was performed by means of modified Marine agar medium and their DNA were extracted and amplified by PCR using universal primers that amplify archaeal 16S rDNA. The amplified archeal DNA fragments were purified, and were subjected to 16S rRNA gene sequencing analysis which was compared to known sequences by a Blast search at NCBI (National Center for Biological Information). Similarity analysis based on 16S rRNA gene sequences of all isolates indicated that the archaeal isolates belong to three different halophilic genera of euryarcheota: Halorubrum, Haloarcula and Halobacterium. These extreme halophilc archaea can be used as a potential source of new therapeutic metabolites and enzymes as well as antibiotic compounds along with novel biotechnological applications.

References

  1. Oren A. Molecular ecology of extremely halophilic Archaea and Bacteria. FEMS Microbiology Ecology. 2002; 39 (1):1-7.
  2. Eimanifar A, Mohebbi F. Urmia Lake (northwest Iran): a brief review. Saline systems. 2007; 3 (5):1-8
  3. Kanekar P, Kanekar S, Kelkar A, Dhakephalkar P. Halophiles–Taxonomy, diversity, physiology and applications. Microorganisms in Environmental Management: Springer; 2012. p. 1-34.
  4. Raghavan T, Furtado I. Occurrence of extremely halophilic Archaea in sediments from the continental shelf of west coast of India. Current Science. 2004; 86 (8):1065-7.
  5. Lizama C, Monteoliva-Sanchez M, Suarez-Garcia A, Rosello-Mora R, Aguilera M, Campos V, et al. Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile. International journal of systematic and evolutionary microbiology. 2002; 52 (1):149-55.
  6. Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. Journal of Computational biology. 2000; 7 (1-2):203-14.
  7. Oren A. Microbial diversity and microbial abundance in salt-saturated brines: why are the waters of hypersaline lakes red? Natural Resources and Environmental Issues. 2009; 15:247.
  8. Oren A. Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. International journal of systematic and evolutionary microbiology. 2012; 62 (2):263-71.
  9. Benlloch S, Acinas S, Anton J, Lopez-Lopez A, Luz S, Rodriguez-Valera F. Archaeal biodiversity in crystallizer ponds from a solar saltern: culture versus PCR. Microbial Ecology. 2001; 41(1):12-9.
  10. Kerkar S. Ecology of hypersaline microorganisms In Marine Microbiology: Facets and Opportunities, Ed. by Ramaiah N., Goa: National Institute of Oceanography; 2004: pp. 37-47.

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