Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method

Document Type : Research(Original) Article

Authors

1 Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Department of Clinical Pharmacy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

3 Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

To develop and evaluate a sensitive, accurate, rapid and reproducible high performance liquid chromatography analytical method for concurrent assay of simvastatin, a hyperlipidemia controlling agent, and citicoline, a psychostimulant agent, a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) used as a reversed stationary phase and mobile phase was water (previously adjusted with phosphoric acid to a pH of 5.5), methanol and acetonitrile (20:20:60) with the flow rate 1.0 ml/min. The ultraviolet detector was set at 247 nm. A linear correlation between each concentration and its own AUC within concentration ranges of 15 to 100 g/ml for citicoline and 7.5 to 50 g/ml for simvastatin with a correlation coefficient 0.9969 for citicoline and 0.994 for simvastatin were produced. The within and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests show an accurate, rapid and robust HPLC-UV method for successful analysis of both simvastatin and citicoline simultaneously.

Keywords

Trends in Pharmaceutical Sciences
Volume 5, Issue 1
March 2019
Pages 41-46

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